Description
Analysis Note The DNA quality and integrity are evaluated using pulsed field gel electrophoresis and visualization after ethidium bromide staining. The DNA is free of RNA and contaminants that might interfere with restriction endonuclease digestion.
Application For use in Southern hybridizations.
Preparation Note Purified by equilibrium buoyant density ultracentrifugation in cesium chloride, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of approx. 2 units per mL. Approx. 20 A260 units per mg DNA. % GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
Specificity 42% GC; Tm = 87 °C

Properties
mol wt mol wt 10 MDa （≥ 16 kb）
storage temp. 2-8°C

Safety
Personal Protective Equipment Eyeshields, Gloves, type N95 （US）, type P1 （EN143） respirator filter
Hazard Codes B
WGK Germany 3

References
reference Marmur, J., et al. J. Mol. Biol. 5, 109, （1962）
  , Sambrook, J., et al. Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY , （1989）, 9.14

 Printable Reviews
Product ReviewsReview This Product
  
  
       

• Z0131-1脫氧核糖核酸緩沖液/DNA Buffer
• Z0081-12′-脫氧胞苷-5′-三磷酸二鈉鹽/三磷酸脫氧胞苷鈉鹽/dCTP，2Na
• Z0072-12′-脫氧腺苷-5′-單磷酸二鈉/脫氧腺苷單磷酸二鈉/脫氧腺苷酸二鈉/dAMP，2Na
• Z0055-15-胞苷二磷酸單鈉鹽/5-胞嘧啶核苷二磷酸單鈉鹽/胞苷-5′-二磷酸單鈉鹽/胞磷膽堿鈉/胞二磷膽堿鈉
• Z0156黃素腺嘌呤二核苷酸二鈉鹽/黃素腺嘌呤二核苷酸/黃素腺嘌呤二核甙酸二鈉/核黃素-5'-腺苷二磷酸二鈉/
• Z0155乙酸-β-萘酯/乙酸-2-萘酯/醋酸-β-萘酯/β-Napthyl acetate
• Z0154乙酸-α-萘酯/乙酸-1-萘酯/醋酸-1-萘酯
• Z0153麥草畏/百草敵/3,6-二氯-2-甲氧基苯甲酸/麥草威/Dicamba
• Z0152茶堿/1,3-二甲基黃嘌呤/Theophylline
• Z0151茶多酚/茶精/綠茶提取物/Tea Polyphenols/PPT