Description
Application Designed for molecular weight determinations on silver stained SDS-PAGE （Laemmli） gels. The proteins supplied in these markers cover the range of molecular weights common to most proteins or their subunits. The markers are formulated to yield a distribution of well-defined bands of approximay equal intensity with low background after electrophoresis and staining using either ProteoSilver™ （PROTSIL1） or ProteoSilver™ Plus （PROTSIL2）.
Other Notes Will yield 9 bands with silver staining:
• α2-Macroglobulin, 180,000 Da
• β-Galactosidase, E. coli, 116,000 Da
• Phosphorylase b, Rabbit Muscle, 97,000 Da
• Serum Albumin, Bovine, 66,000 Da
• Fumarase, Porcine Heart, 48,500 Da
• Carbonic Anhydrase, Bovine Erythrocytes, 29,000 Da
• β-Lactoglobulin, Bovine Milk, 18,400 Da
• α-Lactalbumin, Bovine Milk, 14,200 Da
• Aprotinin, Bovine Lung, 6,500 Da
 
Packaging Vial contains 200 μl of protein markers. Supplied with one vial of 2× Laemmli sample buffer （S3401）.
Reconstitution Dilute the concentrate between 1:15 and 1:30 depending on the amount of protein loaded in the other lanes. Recommended loading volumes are 10 μl per well for mini gels （10 × 10 cm） or 20 μl per well for standard format gels （16 × 18 cm）.
Physical form Solution in 300 mM NaCl, 100 mM DTT, 3 mM NaN3, and 50% glycerol

Properties
form aqueous glycerol solution
usage sufficient for 300 standard gels （16 × 18 cm）
   vial sufficient for 600 mini-gel applications
mol wt mol wt 6,500-180,000 Da
shipped in dry ice
storage temp. −20°C

Safety
Hazard Codes B
WGK Germany 1

References
reference Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685, （1970） Abstract
  Heukeshoven, J. and Dernick, R., Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining. Electrophoresis 6, 103-112, （1985）

 Printable Reviews
Product ReviewsReview This Product
  
  
       

• D0069纖粘連蛋白/纖連蛋白（人血）/纖鏈蛋白/CIG/Fibronectin
• D0068瓊脂糖凝膠4B蛋白A/Protein A-Sepharose 4B, Fast Flow from
• D0067肌紅蛋白/Myoglobin from equine skeletal muscle
• D0066金屬硫蛋白/Metallothionein/MT
• D0065瓊脂糖蛋白A/Protein A–Agarose
• D0064復(fù)合蛋白保護(hù)劑HPD/Protein protectant HPD
• D0063復(fù)合蛋白保護(hù)劑DSP50/Protein protectant DSP50
• D0062復(fù)合蛋白保護(hù)劑DP7/Protein protectant DP7
• D0061復(fù)合蛋白保護(hù)劑DP4/Protein protectant DP4
• D0060蛋白保護(hù)劑AEP-HBC/Protein protectant AEP-HBC