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13817140470
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13817140470時間:2009/12/23 瀏覽次數(shù):1293
1. Nutrient Agar (營養(yǎng)肉汁瓊脂)
Pepton (蛋白胨)
NaCl
Distilled water (蒸餾水) Adjust (調(diào)) pH to 7.0-7.2
[Note]:When c*tion of Bacillus,5mg of to MnSO4.H2O may be added .
It is favorable to promote spore formation .
適用范圍:產(chǎn)氣氣桿菌、糞產(chǎn)堿桿菌、蠟狀芽孢桿菌、蠟狀芽孢桿菌蕈狀變種、地衣形芽孢桿菌、巨大芽孢桿菌、多粘芽孢桿菌、塵埃芽孢桿菌、短小芽孢桿菌、嗜熱脂肪芽孢桿菌、枯草芽孢桿菌、枯草芽孢桿菌深黑變種、蘇云金芽孢桿菌、蘇云金芽孢桿菌蠟螟亞種(青蟲菌)、蘇云金芽孢桿菌戈爾斯德變種、蘇云金芽孢桿菌猝倒亞種、產(chǎn)氨短桿菌、黃色短桿菌、谷氨酸棒狀桿菌、北京棒桿菌、大腸埃希氏菌(大腸桿菌)、銅綠假單胞菌(綠膿桿菌)、凸形假單胞桿菌、熒光假單胞菌、彎曲假單胞菌、惡臭假單胞菌、假單胞桿菌、藤黃八疊球菌、亞黃八疊球菌、尿素八疊球菌、金黃色葡萄球菌、運動發(fā)酵單孢菌
2. Corn Meal Medium (玉米粉培養(yǎng)基)
Maize flour (玉米粉)
Glucose (葡萄糖)
[Note]:Boil the mixture in autoclave at
into 18ⅹ
3. Lactic-bacteria Medium I (乳酸菌培養(yǎng)基 I )
Yeast extract (酵母膏)
Glucose (葡萄糖)
Tomato juice (西紅柿汁) 100ml Tween (吐溫) 80 0.5ml
Distilled water (蒸餾水) 900ml pH 7.0
適用范圍:植物乳桿菌(胚芽乳桿菌)、嗜熱乳酸鏈球菌
4. Lactic-bacteria Midium Ⅱ (乳酸菌培養(yǎng)基 Ⅱ)
Lacto-casein peptone (乳酪蛋白胨)
Yeast extract (酵母膏)
Tween (吐溫) 80
Na-acetate (醋酸鈉)
MgSO4.7H2O
Distilled water (蒸餾水)
適用范圍:植物乳桿菌(胚芽乳桿菌)
5. Peotone Glucose Yeast extract Medium PGY (蛋白胨、酵母膏、葡萄糖培養(yǎng)基)
Peptone(蛋白胨)
Glucose (葡萄糖)
6. Glycerol Agar (甘油瓊脂)
Peptone (蛋白胨)
Glycerol (甘油)
Agar (瓊脂)
7. Rhizobium medium (根瘤菌培養(yǎng)基)AS 9
Yeast eztract (酵母膏)
Mannitol (甘露醇)
Distilled water (蒸餾水) 800ml pH 7.2
[Note]:Soil extract:Suspend
water. Autoclave at
paper, make up volume to 200ml. Resterilize for 20 minutes at
mixed with othringredients and distributed.
(土壤浸提液的制法:取土壤
適用范圍:大豆根瘤菌(慢生型)、豇豆慢生根瘤菌、花生根瘤菌、紫云英根瘤菌、
大豆根瘤菌(快生型)、大豆根瘤菌、豌豆根瘤菌、苜蓿根瘤菌、田菁根瘤菌
10. Mannitol Agar (甘露醇瓊脂)
Yeast extract (酵母膏)
Mannitol (甘露醇)
Distilled water (蒸餾水) 1000ml
8. Glucose Asparagine (葡萄糖、天門冬素瓊脂)
Glucose (葡萄糖)
K2HPO4
pH 7.2-7.4
適用范圍:刺孢小單孢菌絳紅變種、紫色小單孢菌(絳紅小單孢菌)
12. Gause′s Synthetic Agar (高氏合成一號瓊脂)
KNO3
K2HPO4
NaCl
Agar (瓊脂)
pH 7.2-7.4
適用范圍:刺孢小單孢菌絳紅變種、紫色小單孢菌(絳紅小單孢菌)、白黃鏈霉菌、白色鏈霉菌、抗生鏈霉菌、雙重輪絲鏈霉菌、產(chǎn)色鏈霉菌、燼灰鏈霉菌、天藍(lán)色鏈霉菌、滅蚊鏈霉菌、紅霉素鏈霉菌、青色鏈霉菌、球孢鏈霉菌、淺灰鏈霉菌、灰色鏈霉菌、吸水鏈霉菌、淡紫灰鏈霉菌、黃色長孢鏈霉菌、藤黃色鏈霉菌、細(xì)黃鏈霉菌、黑化鏈霉菌、玫瑰色鏈霉菌、華美鏈霉菌、嗜熱鏈霉菌、委內(nèi)瑞拉鏈霉菌、紫色直絲鏈霉菌、紫色鏈霉菌、綠色鏈霉菌
9. Wort Agar (麥芽汁瓊脂)
Dilute the world (without hop) to 12 Brix. Add
diluted word..Melt the agar by heating, then distribute the medium into tubes.
Autoclave at 110 for 30 minutes.
(將發(fā)酵啤酒的原料(未加酒花),稀釋至12柏林,加瓊脂
適用范圍:克魯斯假絲酵母、郎比可假絲酵母、解脂假絲酵母、馬其頓假絲酵母、擬熱帶假絲酵母、粗壯假絲酵母、皺褶假絲酵母、熱帶假絲酵母、產(chǎn)朊假絲酵母、阿舒假囊酵母、白地霉、果香地霉、地霉屬、異常漢遜酵母、異常漢遜酵母變種、阿拉伯糖醇漢遜酵母、施氏漢遜酵母、菅囊酵母、伯頓畢赤酵母、膜醭畢赤酵母、粘紅酵母、小紅酵母、膠紅酵母、深紅酵母、卡爾斯伯酵母、釀酒酵母、椰園釀酒酵母、發(fā)酵性酵母、洛格酵母、羅斯酵母、魯氏酵母多形魯氏酵母、威爾酵母、酵母、路德類酵母、栗酒裂殖酵母、擲抱酵母、貝雷絲孢酵母、皮狀絲孢酵母、糙孢曲霉、無殼曲霉、鮮橙曲霉、紅曲霉、紫紅曲霉、紅色紅曲霉、紅曲霉菌
10. Potato Dextrose Agar PDA (馬鈴薯、葡萄糖瓊脂)
Potato extract (馬鈴薯汁) 1000ml Dextrose(glucose) (葡萄糖)
Agar (瓊脂)
[Note]: Potato extract: Slice potatoes thin. Add 1000ml of water, immediay
to prevent Oxidtion of juice and boil until soft. (approximay 30min.).
Filter through cotton-cloth. Autoclave at
minutes.(注:取去皮馬鈴薯
適用范圍:酵米面假單胞菌(酵米面黃桿菌)、白色鏈霉菌、燼灰鏈霉菌、青色鏈霉 菌、球孢鏈霉菌、灰色鏈霉菌、龜裂鏈霉菌、傘枝梨頭霉、雅致放射毛霉、棒曲霉、
米曲霉、出芽短梗霉、白僵霉、灰葡萄孢、頂頭孢霉、巴西毛殼、長刺毛殼、橄欖包 毛殼、反曲毛殼、琥珀毛殼、圓酵毛殼、束狀刺盤孢、新月彎孢霉、奇異翅孢殼、地
生翅孢殼、串珠鐮孢、尖鐮孢、盤長孢菌(魯保一號)、銀白楊盤長孢、玉蜀黍長蠕孢、深黃被孢霉、小被孢霉、多頭被孢、拉曼被孢霉、葡萄色被孢霉、生香毛霉、卷枝毛霉、兩型孢毛霉、直立毛霉、球孢毛霉、絲球毛霉、大毛霉、多型孢毛霉、總狀毛霉、魯氏毛霉、五通橋毛霉、黑球漆斑菌、露濕漆斑菌、玫煙色擬青霉、棉鈴蟲擬青霉、擬青霉、球形阜孢、白腐菌、少根根霉、華根霉、科恩根霉、戴爾根霉、日本根霉、爪哇根霉、米根霉、點頭根霉、綠穂霉、簇孢匍柄霉、雅致枝霉、康寧木霉、綠色木霉、黃萎輪枝孢、大麗花輪枝孢
11. Czapek′s Agar(察氏瓊脂)
Sucrose (蔗糖)
MgSO4.7H2O
FeSO4.7H2O
Agar (瓊脂)
pH 7.2
適用范圍:紅霉素鏈霉菌、洋蔥曲霉、金頭曲霉、泡盛曲霉、泡盛曲霉煙色變種、亮白曲霉、炭黑曲霉、鹿皮色曲霉、棒曲霉、無花果曲霉、黃柄曲霉、黃曲霉、煙曲霉、構(gòu)巢曲霉、黑曲霉、赭曲霉、米曲霉、寄生曲霉、醬油曲霉、土曲霉、宇佐美曲霉、焦曲霉、雜色曲霉、溫特曲霉、阿達(dá)青霉、產(chǎn)黃青霉、黃綠青霉、桔青霉、頂青霉、園弧青霉、繩狀青霉、灰黃青霉、島青霉、淡紫青霉、特異青霉、赭綠青霉、產(chǎn)紫青霉、婁地青霉、小刺青霉、蕁麻青霉、
12. Concentrated Sucrose Czapek′s (濃糖察氏瓊脂)
Sucrose (蔗糖)
MgSO4.7H2O
FeSO4.4H2O
Agar (瓊脂)
適用范圍:謝瓦曲霉
13. Synthetic Potato Medium (綜合馬鈴薯培養(yǎng)基)
20%Potato extract (馬鈴薯汁)
KH2PO4
Vitanib B1 (硫胺素) Trace (微量) Agar (瓊脂)
pH 6
適用范圍:米曲霉、醬油曲霉、棲土曲霉、宇佐美曲霉、出芽短梗霉、白僵霉、灰葡萄孢、頂頭孢霉、巴西毛客、長刺毛殼、
球毛殼、橄欖包毛殼、反曲毛殼、琥珀毛殼、圓酵毛殼、多主枝孢、束狀刺盤孢、葫蘆科刺盤孢、新月彎孢霉、奇異翅孢殼、地生翅孢殼、串珠鐮孢、蝕脈鐮孢霉、盤長孢菌(魯保一號)、銀白楊盤長孢、瓶毛殼、金龜子綠僵菌、生香毛霉、卷枝毛霉、兩型孢毛霉、直立毛霉、球孢毛霉、絲球毛霉、大毛霉、多型孢毛霉、總狀毛霉、魯氏毛霉、五通橋毛霉、黑球漆斑菌、露濕漆斑菌、玫煙色擬青霉、棉鈴蟲擬青霉、淡紫色擬青霉、擬青霉、白腐菌、少根根霉、根霉屬的菌、華根霉、科恩根霉、戴爾根霉、中國臺灣根霉、日本根霉、爪哇根霉、黑根霉、雪白根霉、少根根霉、米根霉、點頭根霉、三寶壟根霉、根霉屬的種、綠穂霉、康寧木霉、木素木霉、擬康氏木霉、木霉、粉紅木霉、綠色木霉、黃萎輪枝孢、大麗花輪枝孢、雙孢蘑菇、大肥菇、發(fā)光假蜜環(huán)菌、木耳、皺木耳、毛木耳、大禿馬勃、長根菇、金針菇、棘托竹蓀、短裙竹蓀、短裙竹蓀東北變種(暫定)、長裙竹蓀、白鬼筆、無裙竹蓀、寬鱗大孔菌、凸圓靈芝(白芝)、紫芝、靈芝(紅芝,赤芝,日本靈芝,韓國靈芝)、灰樹花、猴頭菌、香菇、粗毛斗菇、潔麗香菇、豹皮菇、玉蕈、真姬菇、安絡(luò)小皮傘菌、鬼毛針、黑柄皮傘菌、尖頂羊肚菌、圓錐羊肚菌、高羊肚菌、黑脈羊肚菌、羊肚菌、滑菇、光帽黃傘、尖鱗黃傘、金頂側(cè)耳、榆黃磨、金頂磨、白黃側(cè)耳、紫孢平菇、姬菇、裂皮側(cè)耳、擬囊體側(cè)耳(鮑魚菇)、真線側(cè)耳短孢變種、真線側(cè)耳、扇形平菇、扁形平菇、佛羅里達(dá)無孢子側(cè)耳、佛羅里達(dá)側(cè)耳、蠔菇、糙皮側(cè)耳、平菇、貝形側(cè)耳、鷹翅菌、肺形側(cè)耳、柳樹菌、樹窩、扁柄側(cè)耳、美味側(cè)耳、紫孢側(cè)耳、粉褶側(cè)耳、粉紅側(cè)耳、鳳尾菇、漏斗狀側(cè)耳、榆干側(cè)耳、大榆磨、側(cè)耳、茯苓、黑菇、煙色紅菇、裂褶菌、雞縱、金耳、黃木耳、銀耳、白木耳、紫晶口磨、銀絲菇、絲蓋小包腳菇、草菇
14. Corn Meal Medium (玉米粉培養(yǎng)基)
Mix corn meal with water [20:100(W/V)] well . Distribute the mixture into
100ml bottles , each bottle contains about 30ml of the mixture . Autoclave at
15. Filter Paper Medium (濾紙培養(yǎng)基)
(NH4)2SO4
MgSO4.7H2O
Distilled water (蒸餾水) 1000ml A strip of filter paper (濾紙條) 6ⅹ
20. Soybean Cake Meal Agar (黃豆餅粉瓊脂)
10% infusion of soybean cake meal (10%黃豆餅粉浸汁) 1000ml
Glucose (葡萄糖)
16. Sawdust, Wheat-bran Medium (木屑、麩皮培養(yǎng)基)
Sawdust of broad leaf tree
Preparation:Mix these two ingredients well . Add appropriate water and blend
(the amount of water added may be becided by kneading with fingers ). Fill the
mixture into broad mouth of bottles . Stick a hole on the filling, put the
cotton pulg in bottle mouth .
殼斗科植物或闊葉樹的木屑
制法:加水適量充分混勻,水量以手捏時指間有水珠為宜,裝瓶并壓緊,在中心處扎一小洞,堵上棉塞,
`17. Pine Block or Pine Sowdust Medium (松木條或松木屑培養(yǎng)基)
1. Cut
the biocks fully absorbing suger solution .Fill the biocks into broad month of
bottle . Park the bottle with paper . Autoclave at
(將10ⅹ2ⅹ1厘米的松木條浸泡在1-2%的蔗糖水中。使木條吸足糖水,裝瓶不宜過緊,用紙包好,
2. Pine Sawdust Medium
Pine Sawdust (松木屑) 75% Rice bran (米糠) 23-24%
Socrose (蔗糖) 1-2% Water (appropriate)水 (適量)
Autoclave at
18. Rice Straw. Dung Medium (稻草、馬糞培養(yǎng)基)
Dried horse dung (or cow dung) (干馬糞或牛糞) 65%
Died straw (干稻草) 35% Urea (尿素) 0.5%
Calcium superhosphate (過磷酸鈣)0.7% Gypsum powder(石膏粉) 1%
Ammonium sulphate (硫酸銨) 0.3%
[Note]: Add appropriate water, pile them up and cause them fermentation.
(水適量進(jìn)行堆積發(fā)酵)
19. Straw Rice Bran Medium (稻草、米糠培養(yǎng)基)
Dried straw (干稻草) 75-80% Rice bran (米糠) 20-25%
[Note]:Select no mouldy straw and cut them about
in fresh water for 12 hr. Allow the straw fully (the amount of water added may
be decided by kneading with fingers. It is right when a drop of water appeared
between fingers). (
[注]:選無霉稻草,切成長約3厘米的小段,用清水浸泡12小時,使充分吸水,然后傾去水,加米糠和水,水量以用手捏時指間有水珠為宜。)
20. Rice Medium (米飯培養(yǎng)基)
Fill
at
water. Autoclave at
(取大米
21. Filter Paper Medium (濾紙條培養(yǎng)基)
(NH4)2SO4
MgSO4.7H2O
Distilled Water (蒸餾水) 1000ml
Strip of filter paper 1ⅹ
[Note]:Siddolve the above ingredients. Distribute the liqid medium into tubes
(each tube contains 1ml of the broth). Autoclave at
put one or two sterilized strips of filter paper into a tube aseptically.
(將上述溶液分配成溶液后分裝入試管,每管1毫升,
22. Yeast Extract-Medium Extract Agar I (酵母膏、麥芽膏瓊脂)
Yeast extract (酵母膏)
Glucose (葡萄糖)
Agar (瓊脂)
適用范圍:紅霉素鏈霉菌
23. Yeast Extract Peptone (酵母膏、蛋白胨瓊脂)
Yeast extract (酵母膏)
Beef extract (牛肉膏)
Agar (瓊脂)
pH 7.0
24. Glucose Yeast Extract Starch Agar (葡萄糖、酵母、淀粉瓊脂)
Glucose (葡萄糖)
Starch (淀粉)
CaCO3
Distilled water (蒸餾水)
25. Wheat Bran Medium (麩皮培養(yǎng)基)
Wheat bran (麩皮)
K2HPO4
Agar (瓊脂)
pH 7.0
26. Yeast Sporulation Medium (酵母菌產(chǎn)生子囊孢子培養(yǎng)基)
(1) Kleyn Medium
KH2PO4 0.012% K2HPO4 0.02%
Sodium acetate (醋酸鈉) 0.5% Glucose (葡萄糖) 0.062%
NaCl 0.062% Peptone (蛋白胨) 0.25%
Biotin (生物素) 2μg% Agar (瓊脂) 2%
Trace element solution (混合鹽類) 1ml/100ml
[Note]: Trace element solution (混合鹽類溶液): MgSO4.7H2O 0.4%, NaCl 0.4%,
CuSO4.5H2O 0.002%, MnSO4.4H2O 0.2%, FeSO4.4H2O 0.2%
(2) Gorodkowa Medium
Glucose (葡萄糖) 0.1% Peptone (蛋白胨) 1%
NaCl 0.5% Agar (瓊脂) 2%
(3) McClary Medium
Glucose (葡萄糖) 0.1% KCl 0.18%
Yeast juice (酵母汁) 0.25% Sodium actate (醋酸鈉) 0.82%
Agar (瓊脂) 1.5%
27. BPY Medium (BPY 培養(yǎng)基)
Beef extract (牛肉膏)
NaCl
Glucose (葡萄糖)
Distilled (蒸餾水) 1000ml pH 7.0
適用范圍:大腸埃希氏菌(大腸桿菌)、惡臭假單胞菌
28. LB Medium (LB 培養(yǎng)基)
Yeast extact (酵母膏)
NaCl
Distilled water (蒸餾水) 1000ml pH 7.0
適用范圍:大腸埃希氏菌(大腸桿菌)
29. Penssay Broth (Penssay 肉湯)
Beef extract (牛肉膏)
Tryptone (胰蛋白胨)
Glucose (葡萄糖)
Distilled water (蒸餾水) 1000ml pH 7.2
30. Blond Agar (血瓊脂培養(yǎng)基)
Peptone (蛋白胨)
NaCl
Sodium citrate (檸檬酸鈉)
Pig heart infusion (豬心浸) 200ml Sleep Blood (羊血) 10%
pH 7.2
[Note]:Preparation of pig brain and pig heart infusion:
(1) Smash up
water up to 1000ml;
(2) Cut
1000ml;
(3) Keep the two infusions at
(4) Cool and filtrate through gauze .
[注]豬心和豬腦浸汁法:(1)新鮮豬心除去心頭和脂肪,攪碎
31. Cellulose﹣decomposing Bacteria Synthetic Medium (纖維細(xì)菌合成培養(yǎng)基)
NaCl
KH2PO4
K2HPO4
(NH4)2SO4
Filter paper strip: one strip (濾紙條:一片) pH 7.0-7.4
[Note]: So;id medium for cellulose-decomposing bacteria: Carboxymethyl
Cellulose 2% Agar 2%. (斜面培養(yǎng)基去濾紙條用2%瓊脂及2%羧甲基纖維素鈉 CMCNa )
32. Clostridium Pasreurianum Synthetic Medium (巴氏梭狀芽孢桿菌合成培養(yǎng)基)
Glucose (葡萄糖) 1% MnSO4 0.001%
KH2PO4 0.05% FeSO4 0.001%
K2HPO4 0.05% Yeast extract(酵母膏) 0.1%
MgSO4.7H2O 0.02% Peptone (蛋白胨) 0.01%
NaCl 0.001% CaCO3 0.5%
Distille water (蒸餾水) 100% pH 7.0
33. Yeast Extract-Medium Extract Agar Ⅱ (酵母膏、麥芽膏瓊脂 Ⅱ)
Yeast extract (酵母膏)
Glucose (葡萄糖)
Distilled water (蒸餾) 1000ml pH 7.3
34. Oatemeal Agar (燕麥粉瓊脂)
Oatemeal(infusion)[燕麥粉浸液]
Trace element solution (跡量鹽溶液)1ml
Distilled water up to (用蒸餾水補(bǔ)充至1000毫升) 1000ml pH 7.2
[Note]:Trace element solution (跡量鹽溶液):
FeSO4.7H2O
ZnSO4.7H2O
40. Calcium Malate Agar (蘋果酸鈣瓊脂)
Calium malate (蘋果酸鈣)
NH4Cl
Agar (瓊脂)
35. Thiobacillus Ferrooxidans Medium (氧化亞鐵硫桿菌培養(yǎng)基)
(NH4)2SO4
KCl
Ca(NO3)2.4H2O
Distilled water (蒸餾水) 1000ml pH 2.0
36. Thiobacillus Thiooxidans Medium (氧化硫硫桿菌培養(yǎng)基)
(NH4)2SO4
CaCl2 (anhydrous) [無水]
FeSO4.7H2O
pH 3.5-4
37. Plasma Substitute Medium (代血漿培養(yǎng)基)
Sucrose (蔗糖) 12.5-15% Peptone (蛋白胨) 0.5%
KH2PO4 0.03% Na2HPO4.12H2O 0.14%
pH 7.0-7.2
38. Skim Milk Medium (脫脂牛奶培養(yǎng)基) ATCC 183
脫脂牛奶
適用范圍:保加利亞乳桿菌、嗜熱乳酸鏈球菌
39. Pea Agar (豌豆瓊脂)
Glucose (葡萄糖) 1% Peptone (蛋白胨) 0.5%
NaCl 0.5% Agar (瓊脂) 2.0%
Pea infusion (counted by NH2) [豌豆浸汁(以NH2計)] 12-14 mg∕100
pH (before sterilization) [消前] 7.2-7.5
[Note]: Preparetion of pea infusion (豌豆浸汁的制法) :
Preparetion
Adjust pH to 4.3-4.5 by using of sulphuric acid. Add 30ml of chloroform for
antisepsis. Plug with rubber stopper and bind up the bottle mouth with gauge
in order to avoid that the stopper fall off due to gas pressure. Place the
bottle at
escape the chloroform. Filter again Distribution and autoclave at
minutes. Store at cool contition .
(稱取白豌豆
The standard quality of pea infusion as following (豌豆浸汁質(zhì)量標(biāo)準(zhǔn)):
Aminonitrogen(氨基酸): 160-200 mg∕100ml
Soluble phosphoruse(溶磷): 400-600 μg∕ml
Color(色澤): auratus,transparent (金黃、透明)
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